drug administration psilocin  (Cayman Chemical)

Journal: eLife

Article Title: Psilocin fosters neuroplasticity in iPSC-derived human cortical neurons

doi: 10.7554/eLife.104006

Figure Lengend Snippet: ( A ) Enrichment in differentially expressed genes associated with synapse formation, neuronal plasticity, and axonogenesis GO terms 1 day after psilocin administration, and the effect 3 days later. ( B ) Psilocin induced within 24 hrs a first wave of upregulation of selected GO genes based on DESeq2 normalized counts. Log2fold changes of each differentially expressed gene between two conditions are shown as dots. Z scores indicate if more genes in the respective GO term are upregulated or downregulated, also indicated by height and color of the bars. ( C ) TPM-normalized mean (red line) of psilocin-induced temporal expression pattern of genes belonging to the indicated GO. The shaded area indicates SD. ( D ) Chord plots showing significant genes appearing in at least four GO terms (of six selected GO terms) 1 day after psilocin administration and appearing in at least three GO terms 3 days after psilocin administration. ( E ). Heat map (z-scaled normalized counts) showing expression of immediate early genes and AMPA/NMDA receptor genes throughout psilocin administration. ( F ) Differentially expressed genes that are up-/downregulated upon psilocin treatment showed a reversed effect upon co-treatment with ketanserin. Mean TPM-normalized expression values are shown (red line), shaded area indicates S.D. BP, biological process; CC, cellular compartment; KEGG, Kyoto Encyclopedia of Genes and Genomes; TPM, transcripts per kilobase million. The significance was assessed using a Wald test. Significance levels against the respective control ns: p-adj.>0.05, *p-adj. ≤ 0.05, **p-adj. ≤ 0.01, ***p-adj. ≤ 0.001.

Article Snippet: To investigate whether psilocin-induced neuroplasticity effects were PKC-mediated, a 1 mM DMSO stock solution of chelerythrine (Cayman Chemical) was diluted 1:1000 to a final concentration of 1 μM chelerythrine (final DMSO concentration = 0.001%).

Techniques: Expressing, Control

Journal: eLife

Article Title: Psilocin fosters neuroplasticity in iPSC-derived human cortical neurons

doi: 10.7554/eLife.104006

Figure Lengend Snippet: ( A ) Heat map (z-scaled normalized counts) showing expression of genes that are grouped in the selected GO term related to ‘cognition’. Genes that are also present in the GO term related to ‘learning and memory’ are stated as ‘present’, indicating great overlay of the genes of the two GO terms. ( B, C ) Psilocin induced within 24 hrs a first ‘shaking’ wave of selected GO genes based on DESeq2 normalized counts. After 3 days those GO genes normalized. ( B ) Log2fold changes of each gene between two conditions are shown as dots. Z scores indicate if more genes in the respective GO term are upregulated or downregulated, indicated by height and color of the bars. ( C ) TPM-normalized mean (red line) of psilocin-induced temporal expression pattern of genes belonging to the indicated GO. The shaded area indicates SD. TPM, transcripts per kilobase million. The significance was assessed using a Wald test. Significance levels against the respective control ns: p-adj.> 0.05, *p-adj. ≤ 0.05, **p-adj. ≤ 0.01, ***p-adj. ≤ 0.001.

Article Snippet: To investigate whether psilocin-induced neuroplasticity effects were PKC-mediated, a 1 mM DMSO stock solution of chelerythrine (Cayman Chemical) was diluted 1:1000 to a final concentration of 1 μM chelerythrine (final DMSO concentration = 0.001%).

Techniques: Expressing, Control